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KMID : 0545120130230101381
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 10 p.1381 ~ p.1385
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Antimicrobial Activity of the Synthetic Peptide Scolopendrasin II from the Centipede Scolopendra subspinipes mutilans
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Kwon Young-Nam
Lee Joon-Ha
Kim In-Woo
Kim Sang-Hee
Yun Eun-Young
Nam Sung-Hee
Ahn Mi-Young
Jeong Mi-Hye
Kang Dong-Chul
Lee In-Hee
Hwang Jae-Sam
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Abstract
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The centipede Scolopendra subpinipes mutilans is a medicinally important arthropod species. However, its transcriptome is not currently available and transcriptome analysis would be useful in providing insight into a molecular level approach. Hence, we performed de novo RNA sequencing of S. subpinipes mutilans using next-generation sequencing. We generated a novel peptide (scolopendrasin II) based on a SVM algorithm, and biochemically evaluated the in vitro antimicrobial activity of scolopendrasin II against various microbes. Scolopendrasin II showed antibacterial activities against gram-positive and -negative bacterial strains, including the yeast Candida albicans and antibiotic-resistant gram-negative bacteria, as determined by a radial diffusion assay and colony count assay without hemolytic activity. In addition, we confirmed that scolopendrasin II bound to the surface of bacteria through a specific interaction with lipoteichoic acid and a lipopolysaccharide, which was one of the bacterial cell-wall components. In conclusion, our results suggest that scolopendrasin II may be useful for developing peptide antibiotics.
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KEYWORD
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Antimicrobial peptide, SVM algorithm, α-Helical, Scolopendra subpinipes mutilans
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